NanoPro 1000 reagents and consumables
NanoPro 1000 reagents and consumables are designed for use on with NanoPro 1000 and must be used in conjunction with Compass Software version 1.8 or higher and compatible firmware.
Assay Kits
| Part # | Description |
|---|---|
| CBS2001 | XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 Includes 5 boxes of capillaries, five 384-well plates with lids, Anolyte, Catholyte, Wash Concentrate, Luminol, and Peroxide XDR and manifold cleaning sponges. |
| CBS404 | HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| CBS403 | Lysis Kit - Bicine/CHAPS Buffer for Charge Assays Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| CBS401 | Lysis Kit - RIPA Buffer for Charge Assays Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| 041-126 | Amplified Rabbit Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-127 | Amplified Mouse Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
Individual Reagents
| Part # | Description |
|---|---|
| 040-655 | Goat-Anti-Mouse-HRP Secondary Antibody HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-656 | Goat-Anti-Rabbit Secondary Antibody HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-081 | Goat-Anti-Rabbit HUX HRP Secondary Antibody HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-474 | Pan Erk Primary Antibody Pan Erk Primary Antibody, 1X stock (1 mL) |
| 040-477 | Phospho-Erk 1/2 Primary Antibody Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-644 | pI Standard Ladder 1 pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-645 | pI Standard Ladder 2 pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-646 | pI Standard Ladder 3 pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-647 | pI Standard Ladder 4 pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-024 | pI Standard, 4.0 pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-025 | pI Standard, 4.2 pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-026 | pI Standard, 4.4 pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-027 | pI Standard, 4.9 pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-028 | pI Standard, 5.5 pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-029 | pI Standard, 6.0 pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-030 | pI Standard, 6.4 pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-031 | pI Standard, 7.0 pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-032 | pI Standard, 7.3 pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-036 | pI Standard, 8.4 pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-790 | pI Standard, 9.7 pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-967 | Premix G2, ampholyte-free separation gradient Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-968 | Premix G2, pH 3–10 separation gradient Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-970 | Premix G2, pH 4–7 (nested) separation gradient Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-969 | Premix G2, pH 4–9 separation gradient Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-972 | Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-973 | Premix G2, Pharmalyte pH 5–8 separation gradient Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-974 | Premix G2, Servalyt pH 5–8 separation gradient Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-337 | Anolyte Refill Anolyte Refill (200 mL) |
| 040-338 | Catholyte Refill Catholyte Refill (200 mL) |
| 041-108 | Wash Concentrate Refill for Peggy or NanoPro 1000 Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 043-379 | Peroxide Peroxide (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 040-652 | Luminol Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-483 | RIPA Lysis Buffer RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-764 | Bicine/CHAPS Lysis Buffer and Sample Diluent Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-510 | DMSO Inhibitor Mix DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-482 | Aqueous Inhibitor Mix Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C. |
| 040-649 | Sample Diluent Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-309 | Antibody Diluent Antibody Diluent (10 mL) |
| 040-793 | Leveling Solution NanoPro Leveling Solution |
Consumables
| Part # | Description |
|---|---|
| CBS700 | Capillaries-Charge Separation (1 pack) for Peggy or NanoPro 1000 Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (1 box of 96 capillaries) |
| CBS701 | Capillaries-Charge Separation (5 pack) for Peggy or NanoPro 1000 Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (5 boxes of 96 capillaries) |
| 040-663 | Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes five 384-well plates and lids. * Must be ordered with p/n 041-528. |
| 041-528 | Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663. |