Advanced Western Blotting Solutions for Targeted Protein Degradation Research
Simple Western assays provides you critical information to run quantitative DC50 curves with a high throughput workflow while providing you reproducible and quantifiable data. Simple Western systems and their automated Western blotting capabilities enhance accuracy, ensure data quality, and deliver results. Our systems integrate easily in PROTAC® screening assays while providing you the flexibility of seamless transferability of methods and an open platform.
Precise, Quantitative Data for the Accurate Determination of DC50 and Dmax Values
Boost your screening workflow with Simple Western systems, while running up to 96 samples in a single run! In this application note, 120 samples were screened in just two overnight runs to provide highly quantitative data which allows for an accurate determination of DC50 and Dmax. Here, off-the-shelf antibodies from Novus Biologicals and PROTAC® degraders from Tocris were used to automatically profile PROTAC®-induced Cereblon neosubstrate degradation.
Figure: Percent IKZF1 and IKZF3 Degradation by Concentration of Degrader or IMiD.
From your peers: Why Simple Western systems?
Bio-Techne Webinar: Targeted Degradation of the Enhancer Lysine Acetyltransferases CBP and P300
Learn how dCBP-1 will be a useful tool for understanding p300/CBP activity at enhancers in both normal and diseased cells from Christopher J. Ott, PhD, Harvard Medical School. Watch on demand
Simple Western on Jess Steps Up the Accuracy on Dose Response
"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."
—Rachel Doidge Ph.D., Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK
Bio-Techne Webinar: Advanced Targeting Protein Degregation Research with Automated Western Blotting
Watch Dr Gary Allenby, CSO at Aurelia Bioscience, who shares how their research team is utilizing automated Western blot systems to rapidly and reproducibly quantify protein knockdown following treatment with Degraders. Watch on demand
Quantitative Simple Western Assays for Targeted Protein Degradation Research – in the Hands of Your Peers
Are you wanting immunoassay-like quantitation for your Westerns with low sample volume? Get picogram-level sensitivity with just 3 µL of starting material! Learn how fully automated Simple Westerns are being used in labs around the world to quantify Degrader-induced protein knockdown. Read Review Article
VALIDATING TARGETS FOR TARGETED PROTEIN DEGRADATION USING dTAG – A COMPREHENSIVE WORKFLOW SOLUTION
dTAG degradation technology offers a generalizable strategy to degrade, in principle, any intracellular protein of interest. The broad applicability that it offers makes this a useful strategy for exploration and validation of targets, particularly in the context of new Degrader development programs. Read White Paper
Brochure: Targeted Protein Degradation Research
Our TPD Research Product Guide highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation research including, Active Degraders, Degrader Building Blocks, Custom Degrader Services, Proteins and Assays for protein degradation. Request Targeted Protein Degradation Product Guide.
Bio-Techne's Complete Solution for PROTAC® & Targeted Protein Degradation
The Bio-Techne family of brands offer a unique portfolio of high-quality reagents, instruments and services for researchers working in the rapidly growing field of Targeted Protein Degradation. Bio-Techne provides a wide range of tools and reagents, including Active Degraders, TAG Degradation Platform (aTAG, dTAG), Degrader Building Blocks, Assays for Protein Degradation, Ubiquitin Proteasome System Proteins and Assays, and Custom Degrader Services. Visit us to learn more about the range of products available for TPD research.
PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license
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Other Targeted Protein Degradation Resources:
Bio-Techne Provides Complete Solutions for Targeted Protein Degradation
Learn More About PROTAC® Degraders & Targeted Protein Degradation with Tocris
Find Ubiquitin-Related Products, TPD, and PROTACs with R&D Systems
Search PROTAC Products at R&D Systems