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Milo antibody database

The Milo antibody database has moved!

Please visit our new Milo Antibody Database on for the latest antibody information.

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at

Milo Antibodies (394)

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Validated Target  Host SpeciesVendorProduct NumberSampleConcentration
α-actininGoatR&D SystemsAF8279KPC cells100 µg/mL
APRILGoatR&D SystemsAF884MOLT-4 cells1:5
Atg7MouseR&D SystemsMAB6608HeLa cells100 µg/mL
ATG7MouseR&D SystemsMAB6608Macrophages1:10
ATMMouseNovus BiologicalsNB100-309(unpublished)100 µg/mL
BAFFGoatR&D SystemsAF124MOLT-4 cells1:10
beta-tubulinMouseNovus BiologicalsNBP2-50061Jurkat cells50ug/mL
beta-tubulinMouseNovus BiologicalsNB120-7792Jurkat cells50 ug/mL
betaIII-tubulinMouseR&D SystemsMAB1195neural differentiated iPSCs100ug/mL
CCL3/MIP1GoatR&D SystemsAF-270-NADifferentiated THP-1 treated with LPS and Transport Inhibitors20 g/mL
CCR7RabbitNovus BiologicalsNBP2-58753Jurkat cells100 µg/mL
CD-80 (B7-1)GoatR&D SystemsAF140Dendritic cells100ug/mL
CD11bRabbitNovus BiologicalsNB110-89474(unpublished)1:10
CD209 (DC-SIGN)SheepR&D SystemsAF161Dendritic cells100ug/mL
CD25GoatR&D SystemsAF-223-NATregs100ug/mL
CD29GoatR&D SystemsAF2405KPC cells100 µg/mL
CD4GoatR&D SystemsAF-379-NAPBMCs1:10
CD56 (NCAM-1)GoatR&D SystemsAF2408Differentiated NK cells100 g/mL
CD68RatNovus BiologicalsNBP2-33337RAW264.7 cells100 µg/mL
CD81MouseNovus BiologicalsNBP2-53318Gli36 cells100 µg/mL
CD81*MouseNovus BiologicalsNBP1-44861Gli36 cells100 µg/mL
CD83GoatR&D SystemsAF2044Dendritic cells100ug/mL
CD86 (B7-2)GoatR&D SystemsAF-141-NADendritic cells100ug/mL
CRBNRabbitNovus BiologicalsNBP1-91810HeLa cells100 g/mL
Cyclin D1RabbitR&D SystemsAF4196MCF-7 cells100 µg/mL
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