FluorChem E, M and R compatible dyes
Dye/Method | Light Source | Emission Filter | R | M | E |
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Fluorescent Westerns | |||||
MultiFluor Blue | 475 nm LED | 537/26 nm | ![]() |
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MultiFluor Red | 632 nm LED | 710/40 nm | ![]() |
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MultiFluor Green | 534 nm LED | 607/36 nm | ![]() |
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Cy2 | 475 nm LED | 537/26 nm | ![]() |
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Cy3 | 534 nm LED | 607/36 nm | ![]() |
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Cy5 | 632 nm LED | 710/40 nm | ![]() |
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Cy 5.5 | 632 nm LED | 710/40 nm | ![]() |
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DyLight 488 | 475 nm LED | 537/26 nm | ![]() |
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DyLight 550 | 534 nm LED | 607/36 nm | ![]() |
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DyLight 633 | 632 nm LED | 710/40 nm | ![]() |
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DyLight 650 | 632 nm LED | 710/40 nm | ![]() |
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DyLight 680/680B | 632 nm LED | 710/40 nm | ![]() |
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Alexa Fluor 488 | 475 nm LED | 537/26 nm | ![]() |
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Alexa Fluor 546 | 534 nm LED | 607/36 nm | ![]() |
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Alexa Fluor 555 | 534 nm LED | 607/36 nm | ![]() |
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Alexa Fluor 633 | 632 nm LED | 710/40 nm | ![]() |
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Alexa Fluor 647 | 632 nm LED | 710/40 nm | ![]() |
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Alexa Fluor 680 | 632 nm LED | 710/40 nm | ![]() |
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Fluorescein/FITC | 475 nm LED | 537/26 nm | ![]() |
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IRDye 680/680LT | 632 nm LED | 710/40 nm | ![]() |
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Cy7.5 | 747 nm LED | 835/70 nm | ![]() |
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Alexa 790 | 747 nm LED | 835/70 nm | ![]() |
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IRDye 800 | 747 nm LED | 835/70 nm | ![]() |
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Dylight800 | 747 nm LED | 835/70 nm | ![]() |
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CF770 | 747 nm LED | 835/70 nm | ![]() |
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CF790 | 747 nm LED | 835/70 nm | ![]() |
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Blotting | |||||
Chemiluminescence | None | None | ![]() |
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Nucleic Acid Staining | |||||
Ethidium Bromide | Trans UV | Orange (FCM - 593/40 nm) (FCE - 590/50 nm) |
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SYBR® Green | Trans UV 475 LED (FCM only) |
Green (537/26 nm) | ![]() |
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SYBR® Gold | Trans UV 475 LED (FCM only) |
Green (537/26 nm) | ![]() |
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SYBR® Safe | Trans UV 475 LED (FCM only) |
Green (537/26 nm) | ![]() |
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Protein Staining | |||||
Coomassie Blue | Trans UV with White Conversion Screen | Orange (FCM - 593/40 nm) (FCE - 590/50 nm) |
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Silver Stain | Trans UV with White Conversion Screen | Orange (FCM - 593/40 nm) (FCE - 590/50 nm) |
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SYPRO Ruby | Trans UV | Red (FCM - 607/36 nm) (FCE - 620/40 nm) |
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SYPRO Orange | Trans UV 475 LED (FCM only) |
Orange (FCM - 593/40 nm) (FCE - 590/50 nm) |
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SYPRO Red | Trans UV 302 | Red (FCM - 607/36 nm) (FCE - 620/40 nm) |
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Hoechst Blue | Trans UV with Blue Conversion Screen (FCE only) |
Blue (FCE - 460/40 nm) |
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