> Simple Western Assays > Simple Western Size Assays > Simple Western Size-Based Assay Data
What does size-based Simple western assay data look like?
Simple Western size-based assay data is processed automatically in Compass for SW for you. Sample data is displayed by lane in a virtual blot-like image similar to traditional results with one big exception—not only do you get more information, but you also get it as soon as the assay is complete. Quantitative results such as molecular weight, signal intensity (area), % area, and signal-to-noise for each immunodetected protein are presented in the results table automatically.
If you're more familiar with capillary electrophoresis, you can see your results in a more traditional electropherogram view too.
Detectable Molecular Weight Range
With kits offering sizing ranges from 2 kDa to 440kDa, Simple Western assays support the detection of varying protein sizes. Separation module molecular weight ranges:
- 2-40 kDa
- 12 – 230 kDa
- 66 – 440 kDa
Panel of proteins run using both a 12–230 kDa (A) and a 66–440 kDa (B) pre-filled plate.
Reproducible, Quantitative Size-Based Data
Our Simple Western systems give you the detection and quantitation you need, whether you’re looking for absolute or relative quantitation or wanting to simultaneously quantitate high and low protein concentrations.
Simple Western assays are fully automated by Jess, Abby, Wes, Peggy Sue, or Sally Sue and easily standardized, making your results much more reproducible. And there’s no blotting step, so inconsistencies caused by protein transfer are eliminated, which really improves your quantitation too!
Phosphoinositide 3-kinase (PI3K) expression in an MCF-7 lysate. Ten replicates (n=10) of MCF7 lysate at 1 µg/µL were analyzed using a PI3K-specific primary antibody. The predicted molecular weight of PI3K is 110 kDa. Simple Western assay data reported an average molecular weight of 107 kDa with a 0.5% CV for sizing and an 8.7% CV for signal intensity (area).
From Your Peers
“[Wes] Provides quantitative data, saves time and uses far less sample.” - Yotis Senis, Ph.D., Professor and Chair, University of Birmingham, UK
Assay Dynamic range
Simple Western assays have a linear dynamic range of approximately 3 orders of magnitude, letting you detect proteins over a wider concentration range, not to mention with more accurate quantitation
Dynamic range of detection for GSK-3 alpha. >4 logs dynamic range with Simple Western Vs. 2.5 logs with traditional westerns
From Your Peers
“Wes allows us to measure cell signaling proteins from a single muscle fiber segment by lowering our limit of detection nearly ~20-fold from Western blotting. It also allows for much more precise, reproducible, and faster results to be generated, in addition to far greater ease of operation, analysis, and waste disposal.” - Irene Tobias, Ph.D., Postdoctoral Researcher, CSU Fullerton
Additional Simple Western Resources
- Product Insert, Separation Modules for Jess, Abby and Wes
- Molecular weight determination by electrophoresis of SDS-denatured proteins
- Low Molecular Weight Assay Spec Sheet
- High Molecular Weight Made Simple
- Publication Spotlight on high molecular weight protein assays - Simple Western Advances Cutting-Edge Duchenne Muscular Dystrophy Research