Peggy 試薬と消耗品
Peggy試薬と消耗品はPeggy専用に製造されており、Compassソフトウェアver 1.8以上と互換ファームウェアとあわせて使用してください。
Size Separation Assay Kits
| Part # | Description |
|---|---|
| CBS-01-01 | Rabbit (12-180 kDa) Size Separation Master Kit for Sally or Peggy Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Peggy or Sally, Size Detection Kit-Rabbit for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts. |
| CBS-02-01 | Mouse (12-180 kDa) Size Separation Master Kit for Sally or Peggy Core Kit for Peggy or Sally, 5 boxes of Capillaries (96 capillaries per box), Size Separation Kit for Sally or Peggy, Size Detection Kit-Mouse for Peggy or Sally, and Simple Western Standard 1 (MW ladder spans 12-180 kDa). Reagents for 480 Simple Western data points. Ships in 2 parts. |
| CBS301 | Core Kit for Sally or Peggy Includes Matrix Removal Buffer, Wash Buffer, Running Buffer, Luminol-S, Peroxide, Running Buffer cup, Wash Buffer cup, Matrix Removal cup, 5 Manifold Cleaning Sponges and 5 Plates. Reagents for 480 Simple Western data points. Ships in 2 parts. |
| CBS301S1 | Size Separation Kit for Sally or Peggy Includes Separation Matrix, Stacking Matrix, 2 Fluorescent Standard vials, Standard Resuspension Buffer, Sample Buffer and 8 DTT vials. Reagents for 480 Simple Western data points. Ships in 2 parts. |
| CBS301RD | Size Detection Kit-Rabbit for Sally or Peggy Goat-Anti-Rabbit Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points. |
| CBS301MD | Size Detection Kit-Mouse for Sally or Peggy Goat-Anti-Mouse Secondary HRP Conjugate (ready-to-use reagent) and Antibody Diluent. Reagents for 480 Simple Western data points. |
Charge Separation Assay Kits
| Part # | Description |
|---|---|
| CBS2001 | XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 Includes 5 boxes of capillaries, five 384-well plates with lids, Anolyte, Catholyte, Wash Concentrate, Luminol, and Peroxide XDR and manifold cleaning sponges. |
| CBS404 | HeLa Lysate Controls Kit - Bicine/CHAPS Buffer for Charge Assays Includes 5 vials each of low phospho and high phospho HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (5 µL/vial). Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| CBS403 | Lysis Kit - Bicine/CHAPS Buffer for Charge Assays Includes Bicine/CHAPS Lysis Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix, and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| CBS401 | Lysis Kit - RIPA Buffer for Charge Assays Includes RIPA Buffer, Aqueous Inhibitor, DMSO Inhibitor Mix and Cell Wash Buffer. Ships overnight on dry ice. Upon receipt, store components at 4°C. |
| 041-126 | Amplified Rabbit Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Rabbit-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-127 | Amplified Mouse Secondary Antibody Detection Kit Includes SA-HRP conjugate (50 µL), Secondary Goat-Anti-Mouse-Biotin Conjugate (50 µL); Antibody Diluent included. REAGENT FOR PEGGY OR NANOPRO 1000 USERS ONLY. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| PS-T003 | Peggy System Charge Training Kit Includes a XDR Charge Separation Master Kit for Peggy Sue, Peggy or NanoPro 1000 and additional reagents |
Individual Reagents
| Part # | Description |
|---|---|
| CBS201STD1 | Simple Western Size Standard 1 for Sally, Peggy and Simon Biotinylated Ladder 1 (MW ladder spans 12-180 kDa), Ladder Resuspension Buffer and Streptavidin-HRP. Reagents for 480 Simple Western data points. |
| 041-695 | Single Use FL standard Single Use FL standard-strip of 8 tubes (for use with Simple Western Size Standard 1 MW Ladder 12-180 kDa) |
| 041-247 | Separation Matrix Separation matrix 1 (2.5 mL) |
| 041-248 | Stacking Matrix Stacking matrix (1.5 mL) |
| 041-265 | Simon, Sally, Peggy Sample Buffer Simon, Sally, Peggy 10X Sample Buffer (400 µL) |
| 041-808 | Antibody Diluent Plus Antibody Diluent Plus for size assay (10 mL) for Simon, Sally or Peggy. Ships overnight at room temperature. Antibody Diluent is recommended for dilution of primary and secondary antibodies. Upon receipt, store at 4°C. |
| 041-505 | Running Buffer for Sally or Peggy Running Buffer (140 mL) |
| 041-504 | Matrix Removal Buffer for Sally or Peggy Matrix Removal Buffer (140 mL) |
| 041-586 | Wash Buffer for Sally or Peggy Wash Buffer (170 mL) |
| 043-311 | Luminol-S Luminol-S (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 043-379 | Peroxide Peroxide (2 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. |
| 041-468 | HeLa Lysate Control - Bicine/CHAPS Buffer for Sally, Peggy or Simon Includes 1 vial of HeLa lysate, lysed in Bicine/CHAPS Lysis Buffer (35 µL/vial) |
| 040-655 | Goat-Anti-Mouse-HRP Secondary Antibody HRP-conjugated Goat-Anti-Mouse Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-656 | Goat-Anti-Rabbit Secondary Antibody HRP-conjugated Goat-Anti-Rabbit Secondary Antibody (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-081 | Goat-Anti-Rabbit HUX HRP Secondary Antibody HRP-Conjugated Goat-Anti-Rabbit Secondary Antibody cross-absorbed for minimal cross-reactivity against human antigens (50 µL); Antibody Diluent included. ProteinSimple strongly recommends use of the Antibody Diluent for dilution of primary and secondary antibodies as it has been proven superior to any other buffer system tested. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-474 | Pan Erk Primary Antibody Pan Erk Primary Antibody, 1X stock (1 mL) |
| 040-477 | Phospho-Erk 1/2 Primary Antibody Phospho-Erk 1/2 Primary Antibody, 1X stock (1 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-644 | pI Standard Ladder 1 pI Standard Ladder 1: 4.0, 4.9, 6.0, 6.4, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-645 | pI Standard Ladder 2 pI Standard Ladder 2: 4.2, 4.9, 6.0, 6.4, 7.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-646 | pI Standard Ladder 3 pI Standard Ladder 3: 4.9, 6.0, 6.4, 7.0, 7.3 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-647 | pI Standard Ladder 4 pI Standard Ladder 4: 4.9, 5.5, 6.0 (175 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-024 | pI Standard, 4.0 pI Standard, 4.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-025 | pI Standard, 4.2 pI Standard, 4.2 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-026 | pI Standard, 4.4 pI Standard, 4.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-027 | pI Standard, 4.9 pI Standard, 4.9 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-028 | pI Standard, 5.5 pI Standard, 5.5 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-029 | pI Standard, 6.0 pI Standard, 6.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-030 | pI Standard, 6.4 pI Standard, 6.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-031 | pI Standard, 7.0 pI Standard, 7.0 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-032 | pI Standard, 7.3 pI Standard, 7.3 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 041-036 | pI Standard, 8.4 pI Standard, 8.4 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-790 | pI Standard, 9.7 pI Standard, 9.7 (50 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-967 | Premix G2, ampholyte-free separation gradient Premix G2, ampholyte-free separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-968 | Premix G2, pH 3–10 separation gradient Premix G2, pH 3–10 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-970 | Premix G2, pH 4–7 (nested) separation gradient Premix G2, pH 4–7 (nested) separation gradient, pH 2-4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-969 | Premix G2, pH 4–9 separation gradient Premix G2, pH 4–9 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-971 | Premix G2, pH 5–6 separation gradient Premix G2, pH 5–6 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-972 | Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug Premix G2, pH 5–8 (nested) separation gradient, pH 2–4 plug (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-973 | Premix G2, Pharmalyte pH 5–8 separation gradient Premix G2, Pharmalyte pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-974 | Premix G2, Servalyt pH 5–8 separation gradient Premix G2, Servalyt pH 5–8 separation gradient (1.5 mL); Ships overnight at room temperature. Upon receipt, store at 4°C and in the dark. |
| 040-337 | Anolyte Refill Anolyte Refill (200 mL) |
| 040-338 | Catholyte Refill Catholyte Refill (200 mL) |
| 041-108 | Wash Concentrate Refill for Peggy or NanoPro 1000 Wash Concentrate Refill (200 mL). REAGENT FOR PEGGY or NANOPRO 1000 USERS ONLY. Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-652 | Luminol Luminol (2 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-483 | RIPA Lysis Buffer RIPA Lysis Buffer (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-764 | Bicine/CHAPS Lysis Buffer and Sample Diluent Bicine/CHAPS Lysis Buffer and Sample Diluent (10 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-510 | DMSO Inhibitor Mix DMSO Inhibitor Mix, 50X (200 µL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-482 | Aqueous Inhibitor Mix Aqueous Inhibitor Mix, 25X (400 µL); Ships overnight on dry ice. Upon receipt, store at -20°C. |
| 040-649 | Sample Diluent Sample Diluent (3 mL); Ships overnight at room temperature. Upon receipt, store at 4°C. |
| 040-309 | Antibody Diluent Antibody Diluent (10 mL) |
| 041-428 | DTT for Peggy or Sally Single-use DTT - strip of 8 tubes. For Peggy or Sally. |
| 041-595 | Instrument Cup (set of 3) Includes set of 3 instrument cups |
| 041-270 | Streptavidin-HRP for Simon, Sally, or Peggy Streptavidin-HRP Conjugate (120 µl) (ready-to-use reagent) for use with Simon, Peggy and Sally |
| 041-606 | Pipetting Guide Pipetting guide for Sally, Peggy, Sally Sue or Peggy Sue Size Assay |
Consumables
| Part # | Description |
|---|---|
| MR700 | Capillaries - Size Separation for Peggy/Sally (1 pack) Peggy/Sally Capillaries for size assays (1 box of 96 capillaries) |
| MR701 | Capillaries - Size Separation for Peggy/Sally (5 pack) Peggy/Sally Capillaries for size assays (5 boxes of 96 capillaries) |
| CBS700 | Capillaries-Charge Separation (1 pack) for Peggy or NanoPro 1000 Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (1 box of 96 capillaries) |
| CBS701 | Capillaries-Charge Separation (5 pack) for Peggy or NanoPro 1000 Peggy, Peggy Sue and NanoPro 1000 Capillaries for charge assays (5 boxes of 96 capillaries) |
| 040-663 | Assay Plate/Lid Kit (5-Pack) for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes five 384-well plates and lids. * Must be ordered with p/n 041-528. |
| 041-528 | Sponge Pack for Peggy/Sally, Peggy Sue/Sally Sue, or NanoPro 1000 Includes 6 manifold cleaning sponges. * Must be ordered with p/n 040-663. |