Single-Cell Western technical library

The Milo Single-Cell Western system can analyze multiplexed protein expression patterns in dissociated tissues to identify and quantify cell subtypes contained in heterogeneous tissues. Generating high quality single-cell suspensions from tissues is an important precursor to a Single-Cell Western experiment in order to preserve cell states and relative abundance in downstream analysis. We hope the following resources may be helpful when considering methods for dissociating your tissue of interest.

In this technical note, we demonstrate how diluting antibodies with Milk-Free Antibody Diluent when probing targets with a goat primary antibody delivers improved assay performance on the Milo Single-Cell Western platform.

This technical note will walk you through multiplexing with Milo and provide you with evidence of and guidance for efficient stripping to achieve multiplexing levels that match or exceed those of the most powerful multiplexed flow cytometers.