iCE technical library

For a list of peer-reviewed articles our products have been cited in, check out the publications page.

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Please note that the general Technical Library page may contain additional product categories of interest.

For access to more posters, publications and presentations, please visit the cIEF forum

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Technical Notes

		Simple Westernを用いてハイスループットで糖鎖のキャラクタリゼーション \| Japanese	[概要表示]
		治療用タンパク質の品質、有効性、および安全性を評価する上で、糖鎖（グリカン）の特性解析は非常に重要で、製品のライフサイクルを通してモニタリングされなければなりません。しかしながら、糖構造は複雑な上に、HPLCおよび質量分析などの通常行われる糖鎖分析手法は高度な専門知識を要するため、タンパク質のグリコシル化の分析は困難であることが少なくありません。このテクニカルノートでは、Peggy Sue™を使って糖鎖プロファイルと電荷もしくは分子量の違いによる不均一性を同時にスクリーニングする新たな全自動ハイスループットの方法について説明します。両方(電荷と分子量)の分析方法を一つの装置に組み合わせたことにより、治療用抗体などの糖タンパク質の重要な属性を簡単にスクリーニングすることができます。
		A Guide to Defining pI Marker Parameters in Empower®

		Apparent Molecular Weight on CE-SDS and SDS-PAGE	[概要表示]
		A difference between CE-SDS and SDS-PAGE is that discrepancies in apparent molecular weight (MW) have been observed between the two methods. While CE-SDS and SDS-PAGE are typically not intended for high-resolution MW determination, many of their applications require a reasonable estimate of apparent MW. This Technical Note highlights relevant peer-reviewed publications that address these factors for comparing MW between CE-SDS and SDS-PAGE.
		SimpleSol – a Novel Protein Solubilizer for Capillary Isoelectric Focusing Analysis Technical Note	[概要表示]
		This technical note introduces SimpleSol, a novel and alternative reagent to urea to solubilize proteins for icIEF analysis on iCE and Maurice systems.
		iCE CFR Software Folder and File Overview	[概要表示]
		ProteinSimple iCE CFR software is used for system control, data acquisition and collection of critical audit information. The files used by the iCE CFR software are created using a file format unique to the software. The file types created by the iCE CFR software have the extensions ICF, CAL, DAT, BCH, IEF and ITF. This document provides file type descriptions and file locations for iCE CFR software V3 and V4. Recommendations for data backup are also provided.
		Speeding Up the iCE3 System Workflow with iCE CFR Software