Multiplex to the Max with Single-Cell Westerns
Want to measure expression of multiple proteins in each single cell in your sample? Milo can multiplex 12+ targets simultaneously and can achieve multiplexing levels that match or exceed the most powerful multiplexed flow cytometers.
Single-Cell Westerns with Milo™ allow you to unlock the single-cell proteome and measure changes in protein expression heterogeneity that aren’t possible with other techniques. Whether you need to conserve limited samples, compare relative abundance of protein isoforms or a phosphorylated form to its total abundance, assess the expression of proteins with a similar molecular weight, or just want to confirm a result with a different antibody, Milo is a powerful solution that lets you do all of this at the single-cell level. Make the most of your research with multiplexing on Milo.
Multiplex on Milo using one or more of the following strategies:
- Size-based multiplexing: Use the SDS-PAGE separation to resolve proteins that differ in molecular weight
- Spectral multiplexing: Use antibodies labeled with distinct fluorophores and image them in different spectral channels on your microarray scanner.
- Stripping & re-probing: Since your sample is covalently bound into the gel after the SDS-PAGE separation and UV light exposure, antibodies can be stripped off with minimal sample losses. This enables multiple rounds of stripping & reprobing on Milo – something that isn’t typically possible with conventional Westerns which rely on weaker electrostatic or hydrophobic interactions to immobilize the sample on a membrane.