A Guide to Western Blot Detection

Chemiluminescence Versus Fluorescence

Picking the appropriate detection method for your Western blot

Since the introduction of Western blotting in the late 1970s, it has become a ubiquitous lab technique, enabling the analysis of protein expression in a wide variety of biological sample types. Whether you’re a Western blot expert or just getting started, understanding the pros and cons of the available chemiluminescence- or fluorescence-based signal detection methods will let you make the right choice for your Western blot experiments.

In this technical note, we review common detection methods used in Western blotting to help you understand your options, improve your workflow and maximize your data, and how FluorChem imagers can give you maximum flexibility in your applications.