Immunoassay Investigation: What are ELISAs really costing you?

Your Guide to Understanding Time and Per Sample Estimates for Your Research

ELISAs are perceived as an economical and straightforward way to detect the presence of a target in a sample. However, multiple, manual wash steps and tedious reagent additions increase your time to result and can introduce human error, which will impact assay reproducibility. We outline a cost-benefit analysis of your workflow to determine time and money savings that the Ella automated immunoassay platform can deliver for your research.

How reliable is your technique?

Whether you just want to repeat an experiment in-house or transfer your immunoassay to other labs, sites or a CRO, you’ll want to get the same reproducible results across multiple users and multiple sites. Achieving good immunoassay reproducibility is tricky with all the multiple, manual steps of traditional ELISAs. Pipetting error and minor protocol variations can introduce variability to your immunoassay.

Because Simple Plex assays run on Ella are precisely controlled in the cartridge, there's no chance of pipetting error. You'll get the same reproducible answers across multiple users and multiple sites. With Ella, factory calibrated standard curves come with each cartridge, eliminating the need to prepare your own standard curve in non-GMP settings, saving you significant time and cost. Simply put, you'll get reliable, consistent answers anywhere, by anyone on any Ella.

How does Ella compare to traditional ELISAs?

How much hands-on time do you spend setting up your experiment, performing washes, antibody incubations and detection? Are you tired of splitting your time between running assays in the lab and analyzing answers at your desk? With Ella, you simply add your sample and buffers to the Simple Plex cartridge, insert the cartridge and start your run. There's only 10–15 minutes of hands-on assay prep time, and she gives you fully analyzed answers in an hour!

Simple Plex versus ELISA Time Savings

We compared the cost per reportable result between a 72x1 (72 samples/1 analyte) Simple Plex assay run on Ella and a traditional 96-well plate ELISA. Factory calibrated standard curves come with each Simple Plex assay cartridge and each sample runs in triplicate, letting you maximize the available positions for your sample. With traditional ELISAs you need to allocate precious space on your plate for standard curves and each sample needs to be run multiple times. Even just comparing duplicate results from traditional ELISAs with Ella, you earn a cost saving of $2.62 per result. Maximize your results with less hands-on time, results in triplicate and cost per sample savings to boot!

Simple Plex versus ELISA Cost Savings

Little sample, big results

Do you want to get more data from your precious sample? Traditional ELISAs can need up to 300 µL of sample to generate triplicate data, with Simple Plex assays on Ella you get triplicate data results with just 25 µL of your precious sample. Ella uses fluorescence detection so she also stretches what you can do with your samples, giving you less assay variability, better sensitivity, a 3–5 log dynamic range and low single-digit CVs. You can use our 32x4 (32 samples/4 analytes) and 16x4 (16 samples/4 analytes) formats to simultaneously run up to four analytes per run and get up to 128 triplicate data results in under 90 minutes.

Want to learn more about Ella?

Watch the video to see the step by step process, from sample loading to analyzed data.

If you're curious about pricing, just send us your request.